Bradford micro-assay

Bradford micro-assay is a technique which is used to determine the total protein concentration in an unknown sample. The presence of protein is determined by a dye named, Comassie Brilliant Blue G250 (Bradford Reagent) which favorably binds to the basic amino acid residues like arginine and lysine. Upon binding, the dye turns blue in the presence of protein or may appear brown in color if the protein is absent in the solution. The quantity of protein is analyzed with the help of a spectrophotometer which measures the quantity of protein by measuring its transmission properties. To analyze the quantity of protein, a standard curve with known amount of protein content is prepared first. The standard curve is a smooth line and by locating the value obtained from unknown sample, we will get the value of the protein content in the unknown sample.


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